Prevalence of Cryptosporidiosis and Molecular Characterization of Cryptosporidium spp. in Calves in Erzurum

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GÜVEN E., AVCIOĞLU H., BALKAYA İ., HAYIRLI A., Kar S., Karaer Z.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.19, sa.6, ss.969-974, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 6
  • Basım Tarihi: 2013
  • Doi Numarası: 10.9775/kvfd.2013.9187
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.969-974
  • Anahtar Kelimeler: Calf, Cryptosporidium, Nested-PCR, SSU rRNA, Erzurum, DAIRY-CATTLE, RISK-FACTORS, INFECTION, GENOTYPES, PARVUM, OOCYSTS, HUMANS, STATES, FARMS, AGE
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study was conducted to determine the prevalence of cryptosporidiosis and to identify Cryptosporidium species found in preweaned calves, in Erzurum, Turkey. Fecal samples were collected from 307 calves up to one month old from 5 dairy farms. Genomic DNA was obtained by DNA extraction (QIAamp DNA Stool kit). The prevalence of cryptosporidiosis was determined based on identification through a nested PCR protocol to amplify fragments of the Cryptosporidium SSU rRNA gene. 3.9% of calves were positive for Cryptosporidium. Calves that were subjected to traditional herd management, were female, aged 2 weeks, and had watery feces were affected by the disease at a greater incidence than those were subjected to planned herd surveillance program, were males, were older than 3 weeks, and had firm feces. DNA sequence analysis of the SSU rRNA gene on all of the PCR positive samples ascertained that C. parvum was the only species present. Further studies should be performed comprehensive fecal analysis for other causative agents for association Cryptosporidium species in calf diarrhea and mortality resulting in economic loss in the region.