Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles

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Cetin M., Aktas Y. E., Vural I., Capan Y., Dogan L., Duman M., ...Daha Fazla

DRUG DELIVERY, cilt.14, sa.8, ss.525-529, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 8
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/10717540701606483
  • Dergi Adı: DRUG DELIVERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.525-529
  • Anahtar Kelimeler: bFGF, chitosan, nanoparticles, SDS-PAGE, TPP, FIBROBLAST-GROWTH-FACTOR, BLOOD-BRAIN-BARRIER, DELIVERY, MICROSPHERES, HYDROGEL, PROTECTION, PARTICLES, ISCHEMIA, FACTOR-2, TISSUE
  • Atatürk Üniversitesi Adresli: Evet

Özet

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.