Akademik Veri Yönetim Sistemi
ROMANIAN BIOTECHNOLOGICAL LETTERS, cilt.16, sa.1, ss.5872-5880, 2011 (SCI-Expanded)
In the present research the usability of the extract prepared from waste loquat (Eriobotrya japonica Lindl.) kernels was investigated as substrate in submerged cultures of moulds (Aspergillus niger and Rhizopus oryzae) and yeasts (Rhodotorula glutinis and Saccharomyces cerevisiae). Loquat kernel was found to be very rich in protein (22.5%) and total carbohydrate (71.2%) contents. The hydrolysate, which was generated by acid hydrolysis (2 N H2SO4) from loquat kernels, was neutralized dried and converted to loquat kernel extract (LKE). In order to appraise the effectiveness of LKE on biomass production, it was compared with malt extract (ME), universally used for the cultivations for moulds and yeasts. ME resulted in slightly higher biomass yields than LKE; however, the test fungi S. cerevisiae grew comparatively well on LKE. The maximum productions of A.niger, R. oryzae, R. glutinis and S. cerevisiae biomasses on LKE were 19.53, 18.26, 8.8 and 3.9 g/L, respectively. LKE was also found to promote the mycelial pellet formation in test moulds. The usability of waste loquat kernels as a general fermentation substrate in submerged culture was demonstrated for the first time in the present study.