Genetic variability in Grapevine virus A from Vitis vinifera L. x Vitis labrusca L. in Sichuan, China

WANG J., XI D., LIU J., CHEN K., LI H., LIU X., ...Daha Fazla

TURKISH JOURNAL OF BIOLOGY, cilt.36, sa.5, ss.542-551, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 5
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3906/biy-1203-23
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.542-551
  • Anahtar Kelimeler: Vitis vinifera L. x Vitis labrusca L., Grapevine virus A, PCR-RFLP, cloning and sequencing, RT-PCR, SHIRAZ DISEASE, VARIANTS, SEQUENCE, GENOME, GRAPEVINE-LEAFROLL-ASSOCIATED-VIRUS-3, IDENTIFICATION, TRANSMISSION, GVA
  • Atatürk Üniversitesi Adresli: Evet

Özet

Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants ( Vitis vinifera L. x Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that the parts of the tested grape plants were specifically infected by mild variants.