In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes

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Turkez H., GEYİKOĞLU F., Dirican E., TATAR A.

CYTOTECHNOLOGY, cilt.64, sa.6, ss.607-612, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s10616-012-9454-1
  • Dergi Adı: CYTOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.607-612
  • Anahtar Kelimeler: Aflatoxin B1, Borax, Chemoprevention, Genotoxicity, Human blood cultures, OXIDATIVE STRESS, BORON-COMPOUNDS, HUMAN BLOOD, BORIC-ACID, LIPID-PEROXIDATION, DIETARY BORON, RAT-LIVER, GENOTOXICITY, B-1, DNA
  • Atatürk Üniversitesi Adresli: Evet

Özet

A common dietary contaminant, aflatoxin B1 (AFB1), has been shown to be a potent mutagen and carcinogen in humans and many animal species. Since the eradication of AFB1 contamination in agricultural products has been rare, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boron compounds like borax (BX) and boric acid are the major components of industry and their antioxidant role has recently been reported. In the present report, we evaluated the capability of BX to inhibit the rate of micronucleus (MN) and sister chromatid exchange (SCE) formations induced by AFB1. There were significant increases (P < 0.05) in both SCE and MN frequencies of cultures treated with AFB1 (3.12 ppm) as compared to controls. However, co-application of BX (1, 2 and 5 ppm) and AFB1 resulted in decreases of SCE and MN rates as compared to the group treated with AFB1 alone. Borax gave 30-50 % protection against AFB1 induced SCEs and MNs. In conclusion, the support of borax was especially useful in aflatoxin-toxicated blood tissue. Thus, the risk on target tissues of AFB1 could be reduced and ensured early recovery from its toxicity.