Cobalt-induced retrotransposon polymorphism and humic acid protection on maize genome

Yigider E., TAŞPINAR M. S., AYDIN M., AĞAR G.

BIOLOGIA FUTURA, cilt.71, ss.123-130, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s42977-020-00001-z
  • Dergi Adı: BIOLOGIA FUTURA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.123-130
  • Anahtar Kelimeler: Cobalt, LTR retrotransposon polymorphisms, Genomic template stability, Humic acid, IRAP, REMAP, DNA METHYLATION, MITOMYCIN-C, GENOTOXICITY, METABOLISM, ACTIVATION, RESPONSES, TOXICITY, PROMOTER, NICKEL, ASSAY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Retrotransposon activity and genomic template stability (GTS) are one of the most significant rearranging mechanisms in environmental stress. Therefore, in this study, it is aimed to elucidate effecting of Cobalt (Co) on the instability of genomes and Long Terminal Repeat retrotransposon polymorphism in Zea mays and whether humic acid (HA) has any role on these parameters. For this purpose, Retrotransposon-microsatellite amplified polymorphism (REMAP) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers were applied to evaluate retrotransposon polymorphism and the GTS levels. It was found that IRAP and REMAP primers generate unique polymorphic band structures on maize plants treated with various doses of Co. Retrotransposon polymorphism increased and GTS decreased while increasing Co concentration. On the other hand, there was a reduction in negative effects of Co on retrotransposon GTS and polymorphism after treatment with HA. The results indicate that HA may be used effectively for the protection of maize seedlings from the destructive effects of Co toxicity.