AS INDICATOR INSECT GROUPS: DETERMINATION OF SUB-SPECIES OF SOME BACTERIA IN NEPA SPP. GENUS’ GUT MICROBIOTA


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Bektaş M., Orhan F., Barış Ö.

INTERNATIONAL BLACK SEA COASTLINE COUNTRIES SCIENTIFIC RESEARCH SYMPOSIUM – VI , Giresun, Turkey, 28 - 30 April 2021, pp.434-441

  • Publication Type: Conference Paper / Full Text
  • City: Giresun
  • Country: Turkey
  • Page Numbers: pp.434-441
  • Ataturk University Affiliated: Yes

Abstract

Fresh water is an essential resource maintaining human life and other organisms in the environment to sustain the balance of nature. Aquatic communities are ideal indicators of environmental scale. In environment, each organism needs to deal with different environmental stressors which disturb their gut microbiota.  Water scorpions can tolerate pollution for a certain dimension. This investigation was aimed to reveal some bacterial subspecies that it was on biotechnological importance using gut structures of a water scorpion. Therefore, Nepa spp. is thought that an important model organism used in this ecological study the genus, is being named water scorpions, are heteropteran bugs of the family Nepidae. All nepids penetrate through the water surface for breathing. Although all species in this aquatic family are referred to as water scorpions. Adults stage of Nepa spp. was collected from their natural habitat in Erzurum Wetlands (Erzurum, Turkey) and live individuals was brought to the laboratory. After surface were applicated to disinfection, digestive structure of the insect was separated by dissection in a sterile environment. It was placed on sterile tubes then it was sent for metagenomics analysis. The amount and purity of the isolated DNA was determined fluorometrically with Qubit. V3-V4 regions of the 16s rRNA gene to be used in species determination were amplified using the SimpliAmp Thermal Cycler with universal 341F- 805R primer sequences. The detected bacterial subspecies are as follows; Salmonella enterica enterica, Pseudomonas syringae pisi, Cutibacterium acnes acnes, Xanthomonas oryzae oryzicola, Rhizobium leguminosarum trifolii, Bacillus thuringiensis kurstaki, Vibrio tapetis tapetis, Klebsiella pneumoniae pneumoniae, Pantoea stewartii stewartii, Pasteurella multocida septica, Propionibacterium phage P100, Staphylococcus aureus aureus, Ainckia indica indica, Lactobacillus buchneri silagei, Photobacterium damselae piscicida, Bacillus cereus anthracis, Clostridium estertheticum estertheticum, Mycoplasma capricolum capripneumoniae, Lactobacillus delbrueckii bulgaricus, Legionella pneumophila pneumophila, Bifidobacterium animalis lactis, Streptomyces peucetius caesius and Paenibacillus larvae larvae. Salmonella enterica enterica, Staphylococcus aureus aureus and Vibrio tapetis tapetis subspecies were abundant identified. Despite of subspecies level, our results seem that positive impetus will be redounded in microbial ecology. It is believed that it is useful data which will be take advantage of bio-monitor and bacterial studies.