Evaluation of Bacillus amyloliquefaciens Strain TV-17C as a Potential Biocontrol Agent Against Postharvest Blue Mold in Oranges


Avan M., Albastawisi E. M., KOTAN R., Firat Ş., Erarslan G.

Applied Fruit Science, cilt.67, sa.6, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 6
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s10341-025-01646-7
  • Dergi Adı: Applied Fruit Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Bacillus amyloliquefaciens, Biological control, Blue mold, Citrus, Fludioxonil, Penicillium italicum, Postharvest disease
  • Atatürk Üniversitesi Adresli: Evet

Özet

Postharvest blue mold, caused by Penicillium italicum Wehmer, is a major constraint in citrus production, leading to considerable economic losses during storage and transportation. In this study, the efficacy of Bacillus amyloliquefaciens strain TV-17C was evaluated as a biological control agent against P. italicum on ‘Washington Navel’ oranges under both in vitro and in vivo conditions. The bacterium significantly inhibited the mycelial growth of P. italicum in dual-culture assays, achieving an inhibition rate of 95.82%. In storage trials conducted in two major citrus-producing regions in Türkiye (Hatay and Mersin), different concentrations of TV-17C (150, 200, and 250 mL/100 L water; 107 CFU mL−1) were tested. The highest efficacy was recorded at 250 mL/100 L, with disease reduction rates of 91.09% and 90.29% in Hatay and Mersin, respectively, comparable to the chemical fungicide fludioxonil (TEACHER 230 SC), which achieved 92.08% and 90.29%, respectively. Notably, no statistically significant difference was found between the performance of B. amyloliquefaciens TV-17C and fludioxonil. These results highlight the potential of B. amyloliquefaciens strain TV-17C as a safe, effective, and environmentally sustainable alternative to chemical fungicides for managing postharvest blue mold in citrus fruits.