Impacts of some antibiotics on human serum paraoxonase 1 activity

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DILEK E. B., KÜFREVİOĞLU Ö. İ., Beydemir S.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.28, sa.4, ss.758-764, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/14756366.2012.681653
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.758-764
  • Anahtar Kelimeler: Paraoxonase, inhibition, drugs, atherosclerosis, organophosphate, LOW-DENSITY-LIPOPROTEIN, ANHYDRASE ISOFORMS I, CARDIOPROTECTIVE ENZYME, OXIDATIVE STRESS, PON1, PURIFICATION, INHIBITION, PLASMA, VITRO, ANTIOXIDANT
  • Atatürk Üniversitesi Adresli: Evet

Özet

Some enzymes are known to be drug target inhibitions of which can be critical for organisms. PON has a critical role to prevent atherogenesis by inhibiting lipid peroxidation. It is well known that paraoxonase 1 (PON1) plays an important function on high-density lipoprotein (HDL) structure to prevent lipid oxidation not only of low-density lipoprotein, but also of HDL itself. We investigated in vitro effects of some medical drugs on PON1 activity from human serum. K-i constants for oxytetracycline hydrochloride, netilmycin sulfate, lincomycin hydrochloride, clindamycin phosphate, and streptomycin sulfate were found as 0.2, 3.73, 18.30, 35.80, and 56.30 mM, respectively. Our results indicate that these commonly used drugs inhibit the activity of the enzyme at very low doses with different inhibition mechanisms.