Determination of residual levels of naloxone, yohimbine, thiophanate, and altrenogest in porcine muscle using QuEChERS with liquid chromatography and triple quadrupole mass spectrometry

Zhang D., Park J., Kim S., Cho S., Cho S., Yi H., ...Daha Fazla

JOURNAL OF SEPARATION SCIENCE, cilt.39, sa.5, ss.835-841, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 5
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jssc.201501206
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.835-841
  • Anahtar Kelimeler: Porcine muscle, Residue analysis, Sample preparation, Triple quadrupole tandem mass spectrometry, Veterinary drugs, VETERINARY DRUG RESIDUES, FOLLICULAR DEVELOPMENT, ANIMAL-TISSUES, METABOLITES, EXTRACTION, EGGS, MILK, MEAT, VALIDATION, ESTRUS
  • Atatürk Üniversitesi Adresli: Hayır

Özet

A quick, easy, cheap, effective, rugged, and safe QuEChERS (method) was used for the simultaneous detection of four veterinary drug residues, namely naloxone, yohimbine, thiophanate, and altrenogest, in porcine muscle, using liquid chromatography with electrospray ionization triple quadrupole tandem mass spectrometry. Because of the unavailability of a suitable internal standard, matrix-matched calibrations were used for quantification, with determination coefficients >= 0.9542. The accuracy (expressed as recovery %) ranged from 60.53 to 83.25%, and the intra-and interday precisions (expressed as relative standard deviations) were <12%. The limits of quantification were 5, 0.5, 2, and 5 ng/g for naloxone, yohimbine, thiophanate, and altrenogest, respectively. Samples purchased from local markets in Seoul, Republic of Korea, revealed no traces of the target analytes. The developed method described herein is sensitive and reliable and can be applied to quantify the tested veterinary drugs in animal tissues.