Akademik Veri Yönetim Sistemi
Molbiyokon’18, İzmir, Türkiye, 5 - 08 Eylül 2018, ss.90
Nano-PCR is a technique that uses the physicochemical properties
of nanomaterials in order to enhance specificity and sensitivity of PCR.
Several studies showed the potential application of nanoparticles (NPs)
for detection of bacterial, parasitic, and viral infections. However, to
our knowledge, NPs have not been used to detect fungal and bacterial
pathogens in a single PCR. Here, we aimed to develop and optimize a
novel multiplex nano-PCR assay for detection of health-threatening
fungal and bacterial strains. For this bpurpose, conserved regions in the
genomes of pathogens were amplified using the species-spesific primers
by multiplex PCR. After confirmation of PCR products by agarose gel
electrophoresis, multiplex nano-PCR was performed in the presence of
gold NPs, quantum dots, and metal NPs. The effect of each nanoparticle
on PCR was evaluated by comparing the PCR cycle number, limit of
detection, and amplification efficiency. In conclusion, we developed
an effective tool serving as spesific, sensitive and faster alternative to
conventional pathogen detection methods