Akademik Veri Yönetim Sistemi
3rd Eurasia Biochemical Approaches & Technologies Congress (3. EBAT), Antalya, Türkiye, 4 - 07 Kasım 2021, ss.94
Cancer, which is the second leading cause of death after heart diseases in developed countries, is responsible for 22.3% of all deaths. Chemotherapy is one of the most effective treatment methods used in cancer treatment. Paclitaxel is a chemotherapy drug mostly used in the treatment of solid tumors. However, severe neuropathic pain syndrome and some other side effects prevent the use of this drug for a long time and in high doses. Many studies are carried out to eliminate these side effects of Paclitaxel. The aim of this study is to investigate the effects of parthenolide (PTL) on the elimination of oxidative stress in rat testicular tissue due to pain caused by paclitaxel (PTX) treatment. For this purpose, 48 male albino Wistar rats (220-295 g body weight) were divided into 6 groups as negative control (NC), positive control (PC), sham group (SG), Treatment 1 (T1), Treatment 2 (T2), and Treatment 3 (T3). Neuropathic pain was induced by intraperitoneal administration of 2 mg/kg PTX (4 doses) to all groups except the NC. To show the formation of pain, the expression of Hcn2 and Kcns1, which are pain-related genes, were examined by Real-Time PCR in rat testicular tissue. An increase in Hcn2 expression and decrease in Kcns1 expression compared to the NC group were illustrated the formation of the pain model. Pain model groups except the PC and SG groups were treated with 1, 2, and 4 mg/kg PTL, respectively, for 14 days. After the treatment of PTL, all rats were sacrificed by decapitation and all testicular tissues were collected. Then, the activity of SOD, CAT, and GPx enzymes was investigated in testicular tissues taken from all groups in case of pain. The activities of the treatment groups including T1, T2, and T3 and the untreated groups including NC, PC, and SG were compared. According to our results, while SOD, CAT and GPx enzyme activities increased during pain, their activities decreased significantly after PTL treatment. In conclusion, it may be thought that PTL, a natural product, could be used to eliminate the oxidative stress that occurs in testicular tissue during chemotherapy with PTX.