ANTIGENOTOXIC AND THE ANTIOXIDANT CAPACITY OF TOTAL EXTRACT OF TWO LICHENS

Anar M., Aslan A., Alpsoy L., KIZIL H. E., AĞAR G.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.25, sa.3, ss.684-691, 2016 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 3
  • Basım Tarihi: 2016
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.684-691
  • Anahtar Kelimeler: Letharia vulpina, Vulpicida pinastri, Antigenotoxicity, Antioxidant, PROTECTIVE ROLE, AFLATOXIN B-1, CLADONIA-RANGIFORMIS, METHANOL EXTRACTS, OXIDATIVE STRESS, USNIC ACID, DAMAGE, L., INDOMETHACIN, GLUTATHIONE
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, we aimed to determine the antigenetoxic and antioxidant capacity of total extract of two lichens against to Aflatoxin B-1 (AFB(1)). For this aim, we obtained the total extracts from Letharia vulpina (LTE) and Vulpicida pinastri (VTE) lichens species. AFB(1) induced oxidative stress and genotoxicity in human lymphocytes in vitro. Our results showed that 5 mu M concentration of AFB(1) increased the frequency micronucleus (MN) and malondialdehyde (MDA) levels, decreased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione (GSH) level. On the other hand, cotreatment of 5, 10 and 20 mu g/ml concentrations of lichen extracts with AFB(1) decreased the frequency of MN and MDA level and activities of SOD, GPx and GSH level increased. The results of the experiments have clearly shown that LTE and VTE have strong antioxidative and antigenotoxic effect in human lymphocytes in vitro.