Detection of<i> Coxiella</i><i> burnetii</i> and characterisation by multiple-locus variable-number tandem repeat analysis in bovine bulk tank milk samples

Yanmaz B., Ozgen E. K.

VETERINARNI MEDICINA, cilt.68, sa.5, ss.185-190, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.17221/87/2022-vetmed
  • Dergi Adı: VETERINARNI MEDICINA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.185-190
  • Anahtar Kelimeler: genotyping, Q fever, raw milk, Turkiye, Q-FEVER INFECTION, DAIRY-CATTLE, MLVA, SEROPREVALENCE, RUMINANTS, DIVERSITY, GENOTYPES, REGION, HERDS, GOATS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Coxiella burnetii is the aetiological agent of Q fever, which is highly prevalent in Turkiye, but informa-tion on the genetic profiles of the bacterium is limited. This study aimed to investigate the presence of C. burnetii in bovine bulk tank milk (BTM) samples by polymerase chain reaction (PCR) and to investigate the genotypes by means of multiple-locus variable-number tandem repeat analysis (MLVA). A total of 25 markets that sold raw cow's milk were analysed by conventional PCR analysis. An MLVA analysis was performed at six loci, namely MS23, MS24, MS27, MS28, MS33, and MS34, to determine the genotypic variations of C. burnetii found in the positive DNA samples. The DNA of C. burnetii was detected in 16% of the BTM samples. The C. burnetii strains identi-fied in the bovine milk samples collected in this study were found to belong to the same genotypic group as those detected in the bovine milk samples gathered in Greece. As a result, both the presence and genotyping studies of C. burnetii on the BTM samples in Turkiye will contribute to the determination of the geographical distribu-tion of the agent.