Determination of microbiological quality and histamine levels in rainbow trouts (Oncorhynchus mykiss)

Creative Commons License

Aksoy A., Sezer C., ATASEVER M., Cetinkaya A., Gulbaz G.

JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.72, sa.4, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.12681/jhvms.29368
  • Dergi Adı: JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Rainbow trout, Microbial quality, Histamine, ELISA, PCR, YERSINIA-ENTEROCOLITICA STRAINS, BIOGENIC-AMINES, LISTERIA-MONOCYTOGENES, FISH, FOOD, IDENTIFICATION, SAFETY, FRESH
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study aims to determine the microbiological quality and histamine levels of fresh-marketed rainbow trouts (Oncorhynchus mykiss). The total mesophilic aerobic bacteria (TMAB), total psychrophilic aerobic bacteria (TPAB), coliform, fecal coliform, Enterobacteriaceae, Pseudomonas, Staphylococcus-Micrococcus and mold-yeast numbers were detected as 4.24, 4.00, 1.10, 0.17, 1.50, 3.05, 0.28 and 3.82 log 10 cfu/g, respectively. Yersinia enterocolitica, Listeria monocytogenes, Escherichia coli O157:H7 and Vibrio spp bacteria were found in 18 (37.5%), 14 (29.1%), 8 (16.6%) and 14 (29.1%) respectively. Twenty-eight out of 51 Listeria isolates (54.9%) were verified through PCR analysis as L. monocytogenes. It was determined that the histamine level in the samples was above the detectable level (>2.5 mg/kg) in 8 of the samples (16.6%)determined using an ELISA method. It was concluded that the detection of foodborne pathogens in fresh-marketed fish samples potentially constitutes a public health hazard.