Akademik Veri Yönetim Sistemi
PAKISTAN JOURNAL OF ZOOLOGY, cilt.51, sa.3, ss.1155-1161, 2019 (SCI-Expanded)
This experiment was conducted to investigate the effects of dietary vitamin-E (alpha-tocopherol acetate), selenium (selenomethionine) and their combination on Se concentration in liver, heart, kidney and breast muscle. In addition, malondialdehyde (MDA) concentration and enzyme activities of glutathioneperoxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in liver, heart, breast and thigh tissues of laying hens were determined in present study. A total of 96 White Lohman laying hens, aged 24 weeks, were randomly divided into 4 groups (n=24), each of which was composed of 6 subgroups. The control group received the basal diet (T-1), treatment groups were fed on the the basal diet plus the three experimental diets included one of the followings: 125 mg/kg vitamin E + basal diet (T-2); 0.5 mg/kg Selenium + basal diet (T-3) and 125 mg/kg vitamin E plus 0.5 mg/kg selenium + basal diet (T-4), respectively. Experiment lasted for 12 weeks. In this study, supplementation of diets with Vit-E, selenium (Se) and their combination significantly increased Se concentration in all examined tissues of treatment groups when compared to the control group. Supplementation of Vit-E, Se and their combination significantly (P<0.05) decreased the concentration of MDA, but, significantly (P<0.05) incrased GSH-Px, SOD and CAT activities in treatment groups. In conclusion, results indicated that Vitamin E and selenium supplementation of laying hen diets could protect these animals from detrimental effect of free radicals by increasing activity of antioxidant enzymes.