Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

Dal, Tuba; Kara, Soner; Cikman, Aytekin; Balkan, Cigdem; Acikgoz, Ziya; Zeybek, Hasan; USLU, Hakan; Durmaz, Riza

doi:10.1016/j.jiph.2018.11.008

Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

Atıf İçin Kopyala

Dal T., Kara S. S., Cikman A., Balkan C. E., Acikgoz Z. C., Zeybek H., ...Daha Fazla

JOURNAL OF INFECTION AND PUBLIC HEALTH, cilt.12, sa.3, ss.337-342, 2019 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 12 Sayı: 3
Basım Tarihi: 2019
Doi Numarası: 10.1016/j.jiph.2018.11.008
Dergi Adı: JOURNAL OF INFECTION AND PUBLIC HEALTH
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.337-342
Anahtar Kelimeler: Brucellosis, Multiplex real-time PCR, Serological diagnosis, Culture, PCR ASSAY
Atatürk Üniversitesi Adresli: Evet

Özet

Objective: Brucellosis is a zoonotic disease with various clinical presentations and early diagnosis is crucial to avoid severe complications. Due to limitations of conventional diagnostic methods, polymerase chain reaction (PCR) based approaches have gained importance in diagnosis. We aimed to evaluate diagnostic value of multiplex real time-PCR (mRT-PCR) in serum samples collected from brucellosis suspected patients by comparision sensitivity of mRT-PCR with those of conventional diagnostic methods.