Investigation of free radical scavenging enzyme activities and lipid peroxidation in liver tissue of zinc deficient rats
ASIAN JOURNAL OF CHEMISTRY, cilt.20, sa.2, ss.1068-1074, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 20 Sayı: 2
- Basım Tarihi: 2008
- Dergi Adı: ASIAN JOURNAL OF CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1068-1074
- Anahtar Kelimeler: lipid peroxidation, malondialdehyde, antioxidant enzymes, zinc, trace element, free radicals, SERUM OXIDANT/ANTIOXIDANT STATUS, SUPEROXIDE-DISMUTASE ACTIVITY, OXIDATIVE STRESS, SKELETAL-MUSCLE, OXYGEN RADICALS, TRACE-ELEMENTS, DIETARY ZINC, ANTIOXIDANT, GLUTATHIONE, DISEASE
- Atatürk Üniversitesi Adresli: Evet
Özet
The aim of this study was to evaluate the lipid peroxidation and free radical scavenging enzyme activities in liver tissue of zinc (Zn)-deficient rats and investigate relationship among these parameters in either group. 16 Male rats with a weight of 35-40 g were used for the experiment. The rats were divided into control (n = 8) and Zn-deficient groups. After 4 weeks of feeding, the rats were killed by cervical dislocation and liver tissues were removed. Biochemical measurements in liver tissue were carried out using a spectrophotometer. Catalase, glutathione peroxidase, glutathione reductase, glutathione S transferase activities, total (enzymatic plus non-enzymatic) superoxide scavenger activity, superoxide dismutase, non-enzymatic superoxide scavenger activity, superoxide dismutase activities and Zn level in the Zn-deficient group were significantly lower than those of the control group, whereas malondialdehyde level was significantly higher than those of the control group. Slightly increased non-enzymatic superoxide scavenger activity was not significantly different from the controls. The results obtained in this study demonstrate that Zn-deficiency causes a decrease in antioxidant defence system and an increase in oxidative stress in liver tissue in rats.