The aim of the present experimental study was to determine and compare the effect of Hippophae rhomnoides L. extract (HRe-1) and of dexpanthenol on the blood flow of a wound region, in rats using xenon-133 (Xe-133) clearance technique. Methods: Burn wounds were made on both thighs of rats and, HRe-1 and dexpanthenol were applied topically on the wound region only in the right thigh for a period of 8 days. The effect of HRe-1 and of dexpanthenol on blood flow of the wound region was assessed before and after their topical application by using the Xe-133 clearance technique. Results: HRe-1 increased significantly blood flow of the wound region (P<0.05). Dexpanthenol showed a smaller increase in blood flow. In conclusion, our results in rats suggest that HRe-1 increases blood flow of the wound area and can be used for the treatment of skin wound healing, preferably than dexpanthenol.