Atıf İçin Kopyala
Aslan K., Kelle K., Yılmaz M. A., Erden Kopar E., Gülçin I.
IV. International Enzyme and Bioprocess Days , Kars, Türkiye, 7 - 09 Eylül 2023, ss.41
-
Yayın Türü:
Bildiri / Özet Bildiri
-
Basıldığı Şehir:
Kars
-
Basıldığı Ülke:
Türkiye
-
Sayfa Sayıları:
ss.41
-
Atatürk Üniversitesi Adresli:
Evet
Özet
“It is the dose that makes the
poison” expressed Paracelsus nearly 550 years ago as all things are poisonous
at altered concentrations[1], [2]. The phenomenon has been admitted by
researchers since then, and today, most of the dangerous and life-threatening
diseases have been treated by the toxins of bacterial/plant sources or venoms
of insects and herptile animals. This well-accepted way of thinking still keeps
its chair in natural product research and serves humankind in many aspects[2].
Cuckoo-pint
(Arum maculatum L.) has popularity all over the world with its
attribution to poisoning characterized by skin irritation, stomachache, swollen
tongue and throat, vomiting, difficulty in breathing, arrhythmia,
hallucinations, and rarely death[3], [4]. On the other hand, the cuckoo-pint
has been accepted as a cheap and tasty food source (after a long-lasting fermentation
process to detoxify), especially in the southern region of Turkey and the Czech
Republic as it is rich in bioactive compounds, such as alkaloids, saponin,
anthocyanidins, protein, fat, carbohydrates, vitamins and minerals[4], [5].
In this
study, phytochemical characterization and determination of the biological
activity of cuckoo-pint extracts were aimed. Ethanol and water-extracted parts
of the cuckoo-pint were first subjected to LC-MS/MS and flavonoid/phenol
analysis[6]. Then, enzyme inhibition studies over
human carbonic anhydrase I and II, acetylcholine esterase, butyrylcholine
esterase, α -amylase were performed, and IC50 values were determined
for each enzyme inhibition[6], [7]. The extracts' antioxidant capacity
and reducing ability were analyzed by DPPH, ABTS radical scavenging assay, and
FRAP, CUPRAC, and Fe+3-reducing assays, respectively[8]. Also, antimicrobial activity
against some pan-resistant strains (Klebsiella pneumeniae, Staphylococcus
aureus, Escherichia coli, Enterococcus faecalis) was investigated by
agar-disk diffusion assay.