A generalized approach for the surface engineering of nanoparticles in suspension for highly efficient hybrid capture of bio-molecules
Nanotechnology 2010: Advanced Materials, CNTs, Particles, Films and Composites - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010, Anaheim, CA, Amerika Birleşik Devletleri, 21 - 24 Haziran 2010, cilt.1, ss.596-599, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Cilt numarası: 1
- Basıldığı Şehir: Anaheim, CA
- Basıldığı Ülke: Amerika Birleşik Devletleri
- Sayfa Sayıları: ss.596-599
- Anahtar Kelimeler: Aminosilane, DNA hybridization, Magnetic bio-separation, Nanoparticles, Surface functionalization, Tri-phasic reverse emulsion
- Atatürk Üniversitesi Adresli: Hayır
Özet
Surface engineering of nanoparticles with hydrophilic surfaces has been carried out in suspension by a strict control of the surface ad-layer of water surrounding the nanoparticles using a generalized strategy called tri-phasic reverse emulsion (TPRE). This approach produces an optimal density of surface amine groups with monolayer patterns on the nanoparticles surfaces. Surface functionalized nanoparticles when chemically conjugated to oligonucleotide (5 NH2-dC6-dT25), the oligonucleotide conjugated bio-nanoparticles exhibited exceptional hybridization efficiency to fluorescence labeled complementary oligonucleotide (dA25). This strategy has overcome the problem associated with the hydrolysis and condensation of aminosilane molecules during the surface functionalization of nanoparticles in suspension and can have improved efficiency in applications ranging from medicine to pharmaceutics to materials science.