RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.10, sa.6, ss.44-49, 2015 (SCI-Expanded)
To determine the role of Bacillus cereus as a potential pathogen in food poisoning, the production of an emetic toxin (cereulide) and enterotoxigenic by B. cereus was isolated in various food sources. Ninety-two strains of B. cereus isolated from milk, cereal, chicken and meat products have been used to study profiles in detail and by polymerase chain reaction (PCR) for presence of toxin genes. B. cereus can cause diarrheal and emetic type of food poisoning but little study has been done on the main toxins of food poisoning caused by B. cereus in Turkey. The objective of this study is to characterize the toxin gene profiles and toxin-producing ability of 92 B. cereus isolates from food samples in Turkey. The detection rate of nheA, hblC, cytK and EM1 genes among all B. cereus strains was 42, 55,4, 14 and 6,5% respectively.