Foliar bio-stimulant application at flower initiation optimizes chickpea productivity and grain quality in rainfed environments


Khan M. A. B., Asif M., Javaid M. M., Rasheed M., Ercisli S., Alrefaei A. F., ...Daha Fazla

BMC PLANT BIOLOGY, cilt.26, sa.1, 2026 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1186/s12870-026-08129-1
  • Dergi Adı: BMC PLANT BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MEDLINE, Directory of Open Access Journals
  • Atatürk Üniversitesi Adresli: Evet

Özet

Bio-stimulants are emerging as effective agents to improve crop performance under abiotic stress, particularly in rain-fed systems. This two-year field study evaluated the effects of foliar-applied bio-stimulant (Actibion) (1250 mL ha(- 1)) composed of various amino acids including aspartic acid (1.21%), glutamic acid (3.14%), proline (1.15%) at different growth stages-flower initiation (FI), pod initiation (PI), and both (FPI) on chickpea (Cicer arietinum L.) growth, yield, and grain quality under rain-fed conditions in Pakistan. Treatments were compared with water spray and an unsprayed control in a randomized complete block design. Foliar application at the flower initiation stage significantly enhanced key growth parameters, increasing plant height by similar to 22%, pod length by similar to 14%, leaf area index by similar to 23%, and chlorophyll content by similar to 19%, relative to control. Seed yield improved by over 50% across both seasons. Grain nutrient contents (N, P, K) and protein concentration also increased significantly with bio-stimulant application at FI. This treatment yielded the highest net economic returns, demonstrating both agronomic and financial benefits. These findings suggest that foliar application of bio-stimulant at flower initiation is a promising, sustainable approach to enhance chickpea productivity and nutritional quality under rain-fed conditions.