Square Wave Voltammetric Determination of Diclofenac in Pharmaceutical Preparations and Human Serum

CILTAS U., YILMAZ B., KABAN S., AKCAY B. K., NAZIK G.

IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH, cilt.14, sa.3, ss.715-722, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2015
  • Dergi Adı: IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.715-722
  • Anahtar Kelimeler: Diclofenac, Cyclic voltammetry, Square wave voltammetry, Pharmaceutical tablet, Serum, PERFORMANCE LIQUID-CHROMATOGRAPHY, NONSTEROIDAL ANTIINFLAMMATORY DRUGS, HUMAN-PLASMA, ELECTROCHEMICAL DETECTION, METABOLITES, URINE, BIOAVAILABILITY, SODIUM, FLUID
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, a simple and reliable square wave voltammetric (SWV) method was developed and validated for determination of diclofenac in pharmaceutical preparations and human serum. The proposed method was based on electrooxidation of diclofenac at platinum electrode in 0.1 M TBAClO(4)/acetonitrile solution. The well-defined two oxidation peaks were observed at 0.87 and 1.27 V, respectively. Calibration curves that were obtained by using current values measured for second peak were linear over the concentration range of 1.5-17.5 mu g mL(-1) and 2-20 mu g mL(-1) in supporting electrolyte and serum, respectively. Precision and accuracy were also checked in all media. Intra-and inter-day precision values for diclofenac were less than 3.64, and accuracy (relative error) was better than 2.49%. Developed method in this study is accurate, precise and can be easily applied to Diclomec, Dicloflam and Voltaren tablets as pharmaceutical preparation. Also, the proposed technique was successfully applied to spiked human serum samples. No electroactive interferences from the endogenous substances were found in human serum.