ASSESSMENT OF GENETIC DIVERSITY IN TOMATO LANDRACES USING ISSR MARKERS

Henareh M., DURSUN A., Mandoulakani B. A., HALİLOĞLU K.

GENETIKA-BELGRADE, cilt.48, sa.1, ss.25-35, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.2298/gensr1601025h
  • Dergi Adı: GENETIKA-BELGRADE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.25-35
  • Anahtar Kelimeler: ISSR, genetic variation, Shannon's information index, Solanum lycopersicum, POPULATION-STRUCTURE, DNA, VARIETIES, PCR
  • Atatürk Üniversitesi Adresli: Evet

Özet

Tomato is one of the most economically important vegetable crops in many parts of the world. Turkey and Iran are the main producers of tomatoes in the world. The objective of this study was to assess the genetic variation of 93 tomato landraces from East Anatolian region of Turkey and North-West of Iran, along with three commercial cultivars using 14 ISSR primers. The percentage of polymorphic loci (PPL) for all primers was 100%. The mean of expected heterozygosity (He) for the primers varied from 0.153 (UBC808) to 0.30 (UBC848). The dendrogram placed the landraces and commercial cultivars into nine groups. The genotypes originating from the same region, often located in the same group or two adjacent groups. The highest likelihood of the data was obtained when population were located into 2 sub-populations (K = 2). These sub-populations had Fst value of 0.16 and 0.21.