The Inhibitory Effect of Propofol on Bovine Lactoperoxidase

ŞİŞECİOĞLU M., ÇANKAYA M., GÜLÇİN İ., ÖZDEMİR H.

PROTEIN AND PEPTIDE LETTERS, cilt.16, sa.1, ss.46-49, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.2174/092986609787049394
  • Dergi Adı: PROTEIN AND PEPTIDE LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.46-49
  • Anahtar Kelimeler: Propofol, lactoperoxidase, enzyme, purification, inhibition, chromatography, ERYTHROCYTES IN-VIVO, CARBONIC-ANHYDRASE, LIVER-MICROSOMES, VITRO, ANTIOXIDANT, RAT, DANTROLENE, KINETICS, SYSTEM
  • Atatürk Üniversitesi Adresli: Evet

Özet

Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro effect of propofol on lactoperoxidase (LPO; E.C. 1.11.1.7) obtained from bovine milk. Lactoperoxidase was purified with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Lactoperoxidase was purified with a yield of 21.6%, a specific activity of 34 EU/mg proteins and 14.7-fold purification. One enzyme unit is defined as the oxidation of 1 mol ABTS per min under the assay condition (25 degrees C, pH: 6.0). To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The effect of propofol on lactoperoxidase were determined using 2,2'-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate. The IC50 value of propofol was found as 15.97 M. Also, K-i constant for propofol was 3.72 M and propofol was found as competitive inhibitor.