Inhibition effects of some antibiotics on the activity of glucose 6-phosphate dehydrogenase enzyme from rainbow trout (Oncorhynchus mykiss Walbaum, 1792) erythrocytes
Turkish Journal of Veterinary and Animal Sciences, cilt.28, ss.675-681, 2004 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28
- Basım Tarihi: 2004
- Dergi Adı: Turkish Journal of Veterinary and Animal Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.675-681
- Atatürk Üniversitesi Adresli: Evet
Özet
Inhibitory effects of some antibiotics on glucose 6-phosphate dehydrogenase from the erythrocytes of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) were investigated. For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was purified 1271.19-fold in a yield of 70.40% by using ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity gel. A constant temperature (+4 degreesC) was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Thiamphenicol, amikacin, gentamicin, and netilmicin were used as antibiotics. All the antibiotics exhibited inhibitory effects on the enzyme. K-i constants for glucose 6-phosphate dehydrogenase were found by means of Lineweaver-Burk graphs. While thiamphenicol and amikacin showed competitive inhibition, gentamicin and netilmicin displayed noncompetitive inhibition. In addition, I-50 values of the antibiotics were determined by plotting activity % vs. [1].