Biosynthesis of Silver nanoparticles and investigation of genotoxic effects and antimicrobial activity

NADAROĞLU H., Alayli A., Ceker S., Ogutcu H., AĞAR G.

INTERNATIONAL JOURNAL OF NANO DIMENSION, cilt.11, sa.2, ss.158-167, 2020 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 2
  • Basım Tarihi: 2020
  • Dergi Adı: INTERNATIONAL JOURNAL OF NANO DIMENSION
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Academic Search Premier
  • Sayfa Sayıları: ss.158-167
  • Anahtar Kelimeler: Antimicrobial Activity, Green Synthesis, Pathogenic Microorganism, Silver Nanopartides, Vinegar, Genotoxic Effect, INTENSIVE-CARE UNITS, ANTIBACTERIAL ACTIVITY, GREEN SYNTHESIS, SCHIFF-BASES, ANTIMUTAGENIC ACTIVITIES, SUSCEPTIBILITY, DERIVATIVES, RELEASE, EXTRACT
  • Atatürk Üniversitesi Adresli: Evet

Özet

Health risk assessment of nanomaterials is a new and important area emerging; obtaining nanoparticles by green synthesis method and performing cytotoxicity, genotoxicity and antimicrobial testing is an important endpoint. In vitro studies for nanoparticles (NPs) obtained by the non-toxic method offer many advantages, such as the study of the bioavailability of nanomaterials to sensitive target cells. It will be useful for investigating the toxic and genotoxic risks associated with nanoparticle exposure. In this study; silver nanoparticles (AgNPs) were synthesized by green synthesis using grape vinegar prepared by ourselves. The resulting Ag NPs were characterized using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and X-ray powder diffraction (XRD) methods and for different AgNPs concentrations in the range of 5-60 nm. The genotoxic effects of AgNPs were investigated using the Sister chromatid exchange (SCE) test and Micronucleus (MN) tests. Furthermore, the antibacterial and antifungal activities of the synthesized compound were tested against some pathogenic bacteria which are causative agents of the disease. As a result; it was found that the synthesized compound showed different degrees of inhibitory effect on the growth of pathogen strains compared to standard antibiotics. The findings are thought to provide clinically useful information in the treatment of many diseases using AgNPs at optimum concentrations (non-genotoxic concentrations).