SCM-198 Can Regulate Autophagy Through the Bax/Bcl-2/TLR4 Pathway to Alleviate Renal Ischemia-Reperfusion Injury

ERASLAN E., BİRCAN B., TANYELİ A., GÜLER M. C., BAYIR Y., ALTUN S.

EUROBIOTECH JOURNAL, cilt.5, sa.4, ss.161-169, 2021 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.2478/ebtj-2021-0025
  • Dergi Adı: EUROBIOTECH JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.161-169
  • Anahtar Kelimeler: SCM-198, Renal ischemia-reperfusion, Renal injury, Inflammatory cytokines, Autophagy, Bax, Bcl-2, ACUTE KIDNEY INJURY, NF-KAPPA-B, LEONURINE, EXPRESSION, PROTECTS, CELLS, INFLAMMATION, MULTICENTER, APOPTOSIS, OUTCOMES
  • Atatürk Üniversitesi Adresli: Evet

Özet

Renal ischemia-reperfusion (I/R) injury is frequently observed in several clinical cases. In this study, we want to investigate that SCM-198 attenuates renal injury in the renal I/R model and find out the possible mechanisms. Wistar albino 40 male rats were classified into four groups (n=10): control, DMSO, I/R, and SCM-198 30 mg/kg. In the group 4, SCM-198 was administered intraperitoneally once at the doses of 30 mg/kg following the reperfusion. Glomerular associated proteins (PCX), tubular damage factors (NGAL, KIM-1), blood urea nitrogen (BUN), serum creatinine, inflammatory cytokines (IL-1 beta, IL-18, and TNF-alpha), Bax/Bcl-2, TLR4, LC3B, and Beclin-1 were evaluated. SCM-198 played an essential role in mitigating kidney damage. SCM-198 alleviated tubular damage and decreased IL-1 beta, IL-18, and TNF-alpha levels. SCM-198 reduced the apoptosis marker Bax/Bcl-2 ratio, immune system protein TLR4, and autophagy proteins LC3B and Beclin-1. In brief, our results support the notion that SCM-198 has protective effects on I/R-induced renal injury. SCM-198 therapy may be a new alternative for the prevention and treatment of renal I/R injury.