Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.30, sa.6, ss.1082-1089, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 30 Sayı: 6
- Basım Tarihi: 2016
- Doi Numarası: 10.1080/13102818.2016.1224980
- Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1082-1089
- Anahtar Kelimeler: Auxin, CRED-RA, DNA methylation, genomic instability, RAPD, SOMATIC EMBRYOGENESIS, MOLECULAR MARKERS, TISSUE-CULTURE, PLANTS, REGENERATION, RAPD, STABILITY, VARIANTS, TRAITS, GROWTH
- Atatürk Üniversitesi Adresli: Evet
Özet
Genetic variations, and especially somaclonal variations, are undesirable in genetic transformation. In this study, random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) markers were used for detection of the variation in calli that were obtained from endosperm-supported mature embryo of rye on Murashige and Skoog (MS) medium containing different auxins (2,4-D, dicamba and picloram) at a range of different concentrations (2, 4, 6, 8, 10 and 12 mg/L). High level of auxins caused a reduction in the genomic template stability (GTS) value. While the highest GTS was observed in the calli maintained on MS medium with 2 mg/L dicamba (98.4%), the calli maintained on MS medium with 12 mg/L picloram were found to show the least GTS (81.7%) when RAPD patterns were analysed. Epigenetic changes were more frequent and variable than genetic changes when compared to RAPD and CRED-RA results. DNA hypermethylation was observed at higher concentrations of 2,4-D and picloram, whereas DNA hypomethylation was observed in dicamba. These results indicate that RAPD and CRED-RA techniques can be used for detection of somaclonal variation in in vitro cultures, which is a fundamental step in plant genetic transformation.