Akademik Veri Yönetim Sistemi
1st INTERNATIONAL CONGRESS ON NATURAL SCIENCES (ICNAS-2021), Erzurum, Türkiye, 10 - 12 Eylül 2021, ss.298-302
The possibility of depletion of fossil fuel resources and the associated environmental
pollution are directing world energy policies towards the development of alternative
energy resources. The demand for energy has been increasing constantly as the
consequence of evolving technology and rapidly growing world population. Cellulose
considered as an alternative form of energy has recently gained significance and
represents millions of dollars for countries that have the opportunity to obtain energy
from it. At the same time, cellulosic biomaterials are attractive since they are both cheap
and abundant. To use this important resource, its stubborn structure must be broken
down. Undoubtedly, the most attractive environment where cellulolytic enzyme is
produced will be the rumen environment so rumen bacteria are regarded as unique for
this job. Therefore, isolation, identification and characterization of cellulolytic rumen
bacteria or determination of the prokaryotic diversity of rumen environment is of great
importance for cellulase production. For this purpose, in this study, cellulase production
was carried out with Paenibacillus validus bacteria isolated from the rumen and the
culture conditions were optimized. As a result of analysis, the optimal conditions were
determined as; 2 g/L yeast exract, pH:6, 48 hours incubation time and temperature
37oC. Under optimized conditions the cellulase enzyme activity was increased
approximately 2-fold (21 U/mL).