Akademik Veri Yönetim Sistemi
LATIN AMERICAN JOURNAL OF PHARMACY, cilt.36, sa.4, ss.740-749, 2017 (SCI-Expanded)
A novel HPLC-DAD method was succesively developed and validated for determination of beta-carotene (beta-C) and retinol (RT) levels in human plasma. While the isocratic elution was preferred for beta-C analysis, RT analysis was carried out by gradient elution. Methods were validated in terms of linearity, accuracy, precision and sensitivity according to the International Conference on Harmonization guideline. The calibration curves for both beta-C and RT were found to be linear over the concentration ranges of 0.5-10 mu g/mL with r = 0.9996 with respect to the proposed methods. The intra and inter-day precision of beta-C and RT in plasma were calculated to be better than 7.89 and 5.02%, respectively. The present methods were successfully applied to three different group of people who are six healthy volunteers, six patients suffered from diffuse histotypes stomach cancer and six patients suffered from intestinal histotypes stomach cancer. Their blood samples were gently obtained at different hour (0, 1, 2.5, and 5 h) just after the oral administration of single dose of beta-C (20 mg). There was no significant difference for both beta-C and RT concentration with increasing times in healthy volunteers, patients with diffuse and intestinal histotype stomach cancer. In addition, we compared the plasma beta-C and RT concentrations in these groups after 0, 1, 2.5, and 5 h after the oral administration of beta-C. Only at 0 h, plasma RT concentrations were significantly low in intestinal histotype stomach cancer patients comparing to healthy volunteers.