Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats

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Celep N. A., GEDİKLİ S.

ACTA HISTOCHEMICA ET CYTOCHEMICA, cilt.56, sa.1, ss.9-19, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1267/ahc.22-00059
  • Dergi Adı: ACTA HISTOCHEMICA ET CYTOCHEMICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS
  • Sayfa Sayıları: ss.9-19
  • Anahtar Kelimeler: cecal ligation perforation, immunohistochemistry, liver, sepsis, silymarin, NITRIC-OXIDE SYNTHASE, NECROSIS-FACTOR-ALPHA, ACUTE KIDNEY INJURY, ACETAMINOPHEN-INDUCED HEPATOTOXICITY, CECAL LIGATION, LIPID-PEROXIDATION, N-ACETYLCYSTEINE, OXIDATIVE STRESS, MOUSE MODEL, ANTIOXIDANT
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study, it was investigated whether silymarin has a protective effect by performing histo-logical, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg, and 200 mg/kg, by oral one hour before the CLP. As an effect of the histological evaluations of the liver tissues, venous congestion, inflammation, and necrosis in the hepatocytes were observed in the CLP group. A situation close to the control group was observed in the Silymarin (SM)100 and SM200 groups. As a result of the immunohistochemical evaluations, inducible nitric oxide synthase (iNOS), cytokeratine (CK)18, Tumor necrosis factor-alpha (TNF-alpha), and interleukine (IL)-6 immunoreactivities were intense in the CLP group. In the biochemical analysis, Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) levels were signifi-cantly increased in the CLP group, while a significant decrease was observed in the treat-ment groups. TNF alpha, IL-1 beta, and IL-6 concentrations were in parallel with histopathological evaluations. In the biochemical analysis, Malondialdehyte (MDA) level increased significantly in the CLP group, but there was a significant decrease in the SM100 and SM200 groups. Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxi-dase (GSH-Px) activities were relatively low in the CLP group. According to these data, it was concluded that using silymarin reduces the existing liver damage in sepsis.