Simultaneous determination of aminopyrine and antipyrine in porcine muscle, milk, and eggs using liquid chromatography with tandem mass spectrometry

Park J., Zhang D., Kim S., Cho S., Cho S., Yi H., ...Daha Fazla

JOURNAL OF SEPARATION SCIENCE, cilt.38, sa.23, ss.4048-4054, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 23
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/jssc.201500920
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4048-4054
  • Anahtar Kelimeler: Aminopyrine, Animal products, Antipyrine, Pyrazolone derivatives, Tandem mass spectrometry, PYRAZOLONE DERIVATIVES, BOVINE MUSCLE, DIPYRONE, RESIDUES, AGRANULOCYTOSIS, METABOLITES, METAMIZOLE
  • Atatürk Üniversitesi Adresli: Hayır

Özet

The concentrations of residual aminopyrine and antipyrine in porcine muscle, milk, and egg samples were analyzed using liquid chromatography with tandem mass spectrometry after undergoing a series of sample pretreatment steps. Owing to an ion suppression effect, matrix-matched calibrations were used for analyte quantitation with determination coefficients (R-2) >= 0.9931. The recovery rates for aminopyrine and antipyrine in various matrices at two spiking levels (5 and 10 ng/g) fell in the range of 60.96-68.87 and 61.87-66.99%, respectively. Meanwhile, the intra-and inter-day precisions (expressed as relative standard deviation) were 1.02-12.95 and 1.71-5.50%, respectively. The method's detection limit (1 ng/g) was very low, thus enabling the detection of low residue levels. The applicability of the developed method was demonstrated with actual market samples and none of the tested analytes was detected in any of the samples.