Effect of betulinic acid administration on TLR-9/NF-kappa B /IL-18 levels in experimental liver injury

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Dokumacioğlu E., Iskender H., Terim Kapakin K. A., Yenice G., Mokthare B., Bolat İ., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.51, sa.3, ss.1544-1553, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3906/sag-2004-184
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1544-1553
  • Anahtar Kelimeler: Acetaminophen, betulinic acid, interleukin-18, nuclear factor kappa B, toll-like receptor-9, NF-KAPPA-B, ACETAMINOPHEN-INDUCED HEPATOTOXICITY, OXIDATIVE STRESS, INFLAMMATION, EXPRESSION, CHITOSAN, DAMAGE, IL-18, NRF2, TLR
  • Atatürk Üniversitesi Adresli: Evet

Özet

Background/aim: Acetaminophen (APAP), used in the composition of thousands of preparations, is the most commonly used analgesic and antipyretic drug. The present study aimed to investigate the potential protective effects of the betulinic acid (BA) treatment through an APAP-induced hepatotoxicity rat model, using inflammatory, biochemical, and histopathological parameters. Materials and methods: The study consisted of four groups: control group, APAP group, BA group, and APAP+BA group. Experimental studies continued for fifteen days. Serum samples were analysed for glucose, total cholesterol (TChol), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), aspartate amino transferase (AST), malondialdehyde (MDA), toll-like receptor-9 (TLR-9), nuclear factor kappa B (NF-kappa B), and interleukin-18 (IL-18). Results: TLR9, IL-18, NF-kappa B, and MDA levels increased significantly in liver injury groups. These increases considerably decreased by the BA treatment. All groups showed immunopositivity for 8-hydroxy-2'-deoxyguanosine (8-OHdG) and interleukin (IL-1 beta) in the hepatocytes, inflammatory cells, and epithelial cells of bile ducts. Conclusion: BA can be used as an effective agent in the prevention and treatment of acute liver diseases due to its inhibitory properties in multiple pathways and its potent antioxidant effects.