Akademik Veri Yönetim Sistemi
ARABIAN JOURNAL OF CHEMISTRY, cilt.18, sa.6, 2025 (SCI-Expanded, Scopus)
Medicinal plants have emerged as valuable inspiration for novel drug discovery owing to their abundant secondary metabolites. This study aimed to further investigate the anti-non-alcoholic steatohepatitis (NASH) potential of secondary metabolites from the traditional Tibetan medicine (TTM) Saxifraga tangutica (S. tangutica). Using medium-pressure liquid chromatography (MPLC) and hydrophilic/reversed-phase liquid chromatography, nine high-purity (>95%) compounds were isolated, including two novel phenolic glycosides: beta-D-galactoside-1,6-bis(3,4,5-trihydroxybenzoate) and -(-)-rhododendron-4'-beta-D-glucoside. Pharmacological evaluation through Oil Red O staining coupled with comprehensive biochemical profiling demonstrated dose-dependent anti-NASH efficacy of the novel glycosides. Subsequent network pharmacology analysis identified the cAMP and sphingolipid signaling pathways as the principal molecular mechanisms. Molecular docking demonstrated that beta-D-galactopyranosyl-1,6-bis(3,4,5-trihydroxybenzoate) exhibited strong binding to HSP90AA1 (-8.65 kcal/mol), and (-)-rhododendrin-4 '-beta-D-glucopyranoside exhibited strong binding to NFE2L2 (-7.88 kcal/mol). Overall, this study further evaluated the medicinal potential of S. tangutica, while these two novel phenolic glycosides are anticipated to contribute valuable molecular backbones and targeting strategies for NASH drug discovery.