Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography

KÖKSAL Z., Kalin R., GÜLÇİN İ., ÖZDEMİR H.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES, cilt.21, sa.1, ss.385-394, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/10942912.2018.1424197
  • Dergi Adı: INTERNATIONAL JOURNAL OF FOOD PROPERTIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.385-394
  • Anahtar Kelimeler: Plant peroxidase, Radish (Raphanus sativus), Turnip (Brassica rapa), Pesticides, Enzyme inhibition, GLUTATHIONE-S-TRANSFERASE, ANHYDRASE ISOENZYMES I, CARBONIC-ANHYDRASE, STEP PURIFICATION, ACETYLCHOLINESTERASE, LACTOPEROXIDASE, BUTYRYLCHOLINESTERASE, DERIVATIVES, ENZYME, ACID
  • Atatürk Üniversitesi Adresli: Evet

Özet

The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2,4-d-acid dimethylamine, fenoxaprop-p-ethyl, glyphosate isopropylamine, haloxyfop-p-methyl, cypermethrin, -cyhalothrin, and dichlorvos on the peroxidase purified from turnip (Brassica rapa L.) and black radish (Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, -cyhalothrin showed the strongest inhibitory effect against turnip peroxidase (K-i: 1.23 x 10(-2)+/- 0.21 x 10(-2)mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase (K-i: 2.14 x 10(-2)+/- 0.08 x 10(-2)mM) as competitive inhibition.