Exopolysaccharide of Anoxybacillus pushchinoensis G11 has antitumor and antibiofilm activities

Genc B., Taşkın M., Adıgüzel A.

ARCHIVES OF MICROBIOLOGY, cilt.203, sa.5, ss.2101-2118, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 203 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s00203-021-02185-x
  • Dergi Adı: ARCHIVES OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.2101-2118
  • Anahtar Kelimeler: Anoxybacillus pushchinoensis, Antitumor, Antibiofilm, Newtonian flow, Sulfamethoxazole
  • Atatürk Üniversitesi Adresli: Evet

Özet

Exopolysaccharides (EPS/EPSs) possess several various applications in the food and pharmaceutical industries. This study was performed to investigate the biological (antibiofilm and antitumor), rheological (temperature, shear rate, and density) and chemical (solubility, carbohydrate and protein content, composition, molecular weight, functional group analysis, thermal analysis, X-ray diffraction pattern and scanning electron microscopy) properties of the EPS, which was purified from the locally isolated thermophilic bacterium Anoxybacillus pushchinoensis G11 (MN720646). EPS was found to have antibiofilm and antitumor [lung (A-549) and colon (Caco-2 and HT-29) cancer] activities. The viscosity of EPS showing Newtonian flow was temperature dependent. As chemical properties, the EPS was found to be a heteropolysaccharide containing arabinose (57%), fructose (26%), glucose (12%), and galactose (5%). EPS contained 93% carbohydrates and 1.08% protein. The molecular weight of EPS was determined as 75.5 kDa. The FTIR analysis confirmed the presence of sulfate ester (band at 1217 cm(-1)), an indication of the antitumor effect. The EPS was semi-crystalline. It could maintain 36% of its weight at 800 degrees C and crystallization and melting temperatures were 221 and 255.6 degrees C. This is the first report on the EPS production potential and the biological activity of A. pushchinoensis.