DNA DAMAGING AND BIOCHEMICAL EFFECTS OF POTASSIUM TETRABORATE

ÇELİKEZEN F. Ç., TÜRKEZ H., Togar B., Izgi M. S.

EXCLI JOURNAL, cilt.13, ss.446-450, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13
  • Basım Tarihi: 2014
  • Dergi Adı: EXCLI JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.446-450
  • Anahtar Kelimeler: Boron, potassium tetraborate, genotoxicity, oxidative stress, antioxidant, BORIC-ACID, BORON-COMPOUNDS, DIETARY BORON, HUMAN BLOOD, IN-VITRO, OXIDATIVE STRESS, CANCER CELLS, GENOTOXICITY, LYMPHOCYTES, TOXICITY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Potassium tetraborate (PTB) is a product resulting from the controlled reaction of potassium hydroxide, water and boric acid (BA). It is used in many areas of industry such as disinfectant, detergent and treatment of contact lenses. PTB is one of the boron compounds which is most commonly used in many areas of industry although very limited information is available concerning its toxicity. Therefore, in this study, it is aimed to determine genetic and biochemical effects of PTB in human blood cell cultures (n=4). PTB was added into culture tubes at various concentrations (0-1280 mu g/ml). Micronucleus (MN) and chromosomal aberration (CA) tests were performed for genotoxic damage influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative effects. The results indicated that all tested concentrations of PTB were found to be non-genotoxic. In addition, low concentrations (1.25, 2.5 and 5 mu g/ml) of PTB caused increases of TAC levels. Furthermore, all concentrations of PTB were not changed the TOS levels in cultured human blood cells. Based on these results, in this study it has been reported for the first time that PTB is not genotoxic and it increases the antioxidant capacity in human peripheral blood lymphocytes.