A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water

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Shao Y., Tian R., Duan J., Wang M., Cao J., Cao Z., ...Daha Fazla

Sensors, cilt.23, sa.2, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.3390/s23020649
  • Dergi Adı: Sensors
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: glyphosate, aptamer, qPCR, SYBR Green I, sensor, ELECTROCHEMICAL SENSOR, SAMPLES, CHROMATOGRAPHY, GLUFOSINATE, METABOLITE, HERBICIDES, PROBE, ASSAY, ACID, AMPA
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2023 by the authors.Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH2-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterwards, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes glyphosate, complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.6 μmol/L–30 mmol/L and the detection limit was set at 0.6 μmol/L. The recoveries in tap water ranged from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The aptamer proposed in this study has good potential for recognizing glyphosate. The detection method combined with qPCR might have good application prospects in detecting and supervising other pesticide residues.