Impact of Some Avermectins on Lactoperoxidase in Bovine Milk

Koksal Z., KALIN R., GÜLÇİN İ., ÖZDEMİR H., ATASEVER A.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES, cilt.19, sa.6, ss.1207-1216, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 6
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/10942912.2015.1076457
  • Dergi Adı: INTERNATIONAL JOURNAL OF FOOD PROPERTIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1207-1216
  • Anahtar Kelimeler: Lactoperoxidase, Enzyme purification, Enzyme inhibition, Avermectins, Affinity chromatography, ANHYDRASE INHIBITORY PROPERTIES, CARBONIC-ANHYDRASE, LIQUID-CHROMATOGRAPHY, SULFONAMIDE DERIVATIVES, EMAMECTIN BENZOATE, ISOENZYMES I, PURIFICATION, ANTIOXIDANT, MOXIDECTIN, ABAMECTIN
  • Atatürk Üniversitesi Adresli: Evet

Özet

Many macrocyclic lactones, including avermectins, are known to be used as a veterinary drug, agricultural pesticides, and insecticides. Lactoperoxidase (EC 1.11.1.7) is one of the peroxidases found in milk. Lactoperoxidase has a natural host defense system against micro-organisms and a natural antimicrobial system. In this study, some macrocyclic lactones, including emamectin-benzoate, doramectin, eprinomectin, abamectin, moxidectin-vetranal, and ivermectin were investigated for in vitro inhibitory effects on the bovine lactoperoxidase enzyme, which was purified using amberlite CG-50 H+ resin and sepharose 4B-L-tyrosine-sulphanamide affinity chromatography 344.6-fold, with a yield of 61.1% and a specific activity of 39.11 EU/mg protein. Emamectin-benzoate, doramectin, eprinomectin, abamectin, moxidectin-vetranal, and ivermectin are also known strong antiparasitary properties. In this study, we demonstrated that avermectins have strong lactoperoxidase inhibitory effects. Of these, the emamectin-benzoate was shown to have the most inhibiting effect against lactoperoxidase with Ki value of 6.82 +/- 2.60 mu M.