Effect of vitamin D<sub>3</sub> and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid

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GEZER A., Aras S. Y., KILIÇ BAYGUTALP N., KARADAĞ SARI E., Bedir G., Mokhtare B., ...Daha Fazla

VETERINARNI MEDICINA, cilt.69, sa.3, ss.84-93, 2024 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 3
  • Basım Tarihi: 2024
  • Doi Numarası: 10.17221/111/2023-vetmed
  • Dergi Adı: VETERINARNI MEDICINA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.84-93
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, the effects of vitamin D-3 (Vit. D) and a stinging nettle [Urtica dioica L. (UD)] extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of Crohn's disease (CD). The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin (SSTR), aquaporin-1 (AQP-1), caspase-3, and tumour necrosis factor-alpha (TNF-a) immunoreactivity in the gastric tissues. In the evaluations, degenerative and necrotic changes and mononuclear cell infiltration areas were observed in the TNBS group, but such changes could be improved with Vit. D and UD applications. The results suggest that the combination of the Vit. D and UD extract may have a protective and therapeutic role in mitigating TNBS-induced damage to the gastric tissues, potentially through the regulation of SSTR, AQP-1, caspase-3, and TNF-a expression. This indicates a promising avenue for further research and the exploration of these compounds in the context of gastrointestinal health.