Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor

TOPAL M., GÜLÇİN İ.

TURKISH JOURNAL OF CHEMISTRY, cilt.38, sa.5, ss.894-902, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/kim-1403-5
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.894-902
  • Anahtar Kelimeler: Carbonic anhydrase I, carbonic anhydrase II, rosmarinic acid, enzyme purification, enzyme inhibition, ERYTHROCYTE ISOZYMES I, ANTIOXIDANT PROPERTIES, VITRO, ENZYME, DERIVATIVES, (3,4-DIHYDROXYPHENYL)(2,3,4-TRIHYDROXYPHENYL)METHANONE, SULFAMIDES, SERIES
  • Atatürk Üniversitesi Adresli: Evet

Özet

Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2-esterase activity gave Ki values of 86.0 mu M and 57.0 mu M, respectively.