Akademik Veri Yönetim Sistemi
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, cilt.29, sa.4, ss.307-312, 2006 (SCI-Expanded)
The present study was planned to investigate the serum disposition kinetics and the pattern of ceftriaxone elimination in milk and urine of lactating ewes (n = 6) following i.v. and i.m. administration. A crossover study was carried out in two phases separated by 15 days. Ceftriaxone was administered at a dosage of 10 mg/kg b.w. in all animals. Serum, milk and urine samples were collected between 0 and 72 h and a modified agar diffusion bioassay method was used to determine the percentage of protein binding and to measure serum, urine and milk concentrations of ceftriaxone. The drug was detected between 5 min and 48 h postdosing. Concentrations of 0.56 (10 h) and 0.52 (12 h), 0.22 (10 h) and 0.19 (12 h), and 2.18 (24 h) and 2.11 (48 h) mu g/mL were measured in serum, milk and urine following i.v. and i.m. administration, respectively. Individual pharmacokinetic parameters were determined by fitting a two-compartment model to the serum and one-compartment open model to the milk concentration-time profiles. After i.v. dosing, the elimination rate constant and elimination half-life were 0.4 +/- 0.05/h and 1.75 +/- 0.02 h, respectively. The volume of distribution at steady state (V-dss) of 0.28 +/- 0.15 L/kg reflected limited extracellular distribution of the drug with total body clearance (Cl-tot) of 0.14 +/- 0.10 L/h/kg. Following i.m. administration, the mean T-max obs, C-max obs, t(1/2el) and AUC values for serum data were: 0.75 h, 23.16 +/- 2.94 mu g/mL, 1.77 +/- 0.24 h and 67.55 +/- 6.51 mu g.h/mL, respectively. For milk the data were: 1.0 h, 8.15 +/- 0.71 mu g/mL, 2.2 +/- 0.34 h and 26.6 +/- 5.14 mu g.h/mL, respectively. The i.m. bioavailability was 83.6% and the binding percentage of ceftriaxone to serum protein was 33%. Concentrations of ceftriaxone in milk produced by clinically normal mammary glands of ewes were consistently lower than in serum; the kinetic value AUC(milk)/AUC(serum) and C-max milk/C-max serum ratios was < 0.4. These low values indicated poor distribution and penetration of ceftriaxone from the bloodstream to the mammary gland of lactating ewes following both routes.