Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, cilt.30, sa.1, ss.49-59, 2000 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 30 Sayı: 1
- Basım Tarihi: 2000
- Doi Numarası: 10.1080/10826060008544944
- Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.49-59
- Atatürk Üniversitesi Adresli: Evet
Özet
Carbonic anhydrase (CA) was purified from bovine erythrocyte plasma membrane and characterized in this study. For this purpose, the blood taken from young:animals was hemolysed, the membrane fraction was separated, and this fraction was repeatedly washed. The enzyme (CA) was removed from the membrane with buffered TritonX-100 (1%); it could be purified at a factor of 22.8 by affinity chromatography.