Akademik Veri Yönetim Sistemi
International Integrated Pollution Prevention and Control 2021, Karabük, Türkiye, 01 Nisan 2021
Insects (hexapods) are the richest group
in terms of species diversity in the World. As a bioindicator in an ecological
spatial, researching microbiota in the digestive system of damaging or useful
hexapods for agricultural production has recently obtained importance for
biological control. It is known that the most important members of hexapod
microbiota are bacteria. Lately, studies based on 16S rRNA gene sequences have
been increasing to identify bacteria with anti-larval effects acquired from
microbiota.
Hexapod collected from different aquatic
environments in and around Erzurum province (Turkey) between May and September
2020. After species identification of the hexapod, it was treated with 70%
alcohol for 5 minutes and surface decontamination was performed to eliminate
possible contaminant microorganisms on the hexapod. The digestive structure was
dissected following aseptic rules in the laboratory. It is purposed to support
the molecular methods in the diagnosis of bacteria with the API (BioMerieux)
system, which is a conventional method. For this reason, the API-20E test,
which is the most suitable API test kit, was applied by taking Gram bacteria
and other biochemical tests into consideration. 16S rDNA amplicons of the
isolates were obtained, sequence analysis was determined, and the derived
sequences were processed and compared with library data
(http://blast.ncbi.nlm.nih.gov/blast.cgi) (Blast analysis). Clinical isolates
were named by evaluating their molecular and conventional data together and
GenBank acceptance numbers were obtained.
Aeromonas
bacteria were the genus with the most samples out of 69 bacteria identified as
a result of this research. Of those of clinical isolates (69), 12 (17%) were
determined. Such as; Aeromonas (4 Aeromonas veronii, 3 Aeromonas allosaccharophila, 4 Aeromonas hydrophila and other Aeromonas rivipollensis). Even though it
is produced in both aerobic and anaerobic environments in isolation processes,
the superiority of aerobic bacteria draws attention. Aeromonas; has been reported to cause infections in humans,
terrestrial and aquatic animals, reptiles and fish. The importance of Aeromonas increases the importance of
extraintestinal infections and sepsis especially in immunocompromised
individuals since they are increasingly included among gastrointestinal
pathogens in recent years.
Our current research is
a preliminary study but more studies and advanced techniques are needed to
define these hexapods microbiota.