Effects of some metal ions on human erythrocyte glutathione reductase: An in vitro study

ÇOBAN T. A., Senturk M., ÇİFTÇİ M., KÜFREVİOĞLU Ö. İ.

PROTEIN AND PEPTIDE LETTERS, cilt.14, sa.10, ss.1027-1030, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 10
  • Basım Tarihi: 2007
  • Doi Numarası: 10.2174/092986607782541060
  • Dergi Adı: PROTEIN AND PEPTIDE LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1027-1030
  • Anahtar Kelimeler: glutathione reductase, human, inhibition, metal ions, PURIFICATION, EXPOSURE, CADMIUM, ENZYME
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, we investigated inhibitory effects of some metal ions on human erythrocyte glutathione reductase. For this purpose, initially human erythrocyte glutathione reductase was purified 1051-fold in a yield of 41% by using 2', 5'-ADP Sepharose 4B affinity gel and Sephadex G-200 gel filtration chromatography. SDS polyacrylamide gel electrophoresis was done in order to control the purification of enzyme. SDS polyacrylamide gel electrophoresis showed a single band for enzyme. A constant temperature (4 degrees C) was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. Hg2+, Cd2+, Pb2+, Cu2+, Fe3+ and Al3+ exhibited inhibitory effects on the enzyme in vitro.