Histopathological and Biochemical Effects of Humic Acid Against Cadmium Toxicity in Brown Trout Gills and Muscles

ALAK G., ATAMANALP M., TOPAL A., ARSLAN H., Oruc E., ALTUN S.

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES, cilt.13, sa.2, ss.315-320, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.4194/1303-2712-v13_2_13
  • Dergi Adı: TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.315-320
  • Anahtar Kelimeler: Toxicity, antioxidant enzyme, brown trout, histopathology, humic acid, ANTIOXIDANT DEFENSE SYSTEM, RAINBOW-TROUT, ONCORHYNCHUS-MYKISS, CARBONIC-ANHYDRASE, LIPID-PEROXIDATION, SEA-BASS, LIVER, CATALASE, WALBAUM, TISSUE
  • Atatürk Üniversitesi Adresli: Evet

Özet

It was biochemically and histopathologically investigated whether humic acid (HA) has protective effects on cadmium (Cd) toxicity on muscle and gills of brown trout (Salmo trutta fario Linnaeus, 1792). The brown trout were exposed to cadmium (2 ppm) and/or humic acid (5 ppm). For this purpose, levels of glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) was investigated in muscle and gills tissues of brown trout. The activities of GPx and SOD in the tissues of fish exposed to Cd was significantly lower than the control groups (P<0.05). MDA levels did not increase in the groups exposed to cadmium (P>0.05). Hovewer, humic acid did not affect biochemical damage in cadmium group. Cd caused a significant increase in histopathological changes in muscle and gills tissues, but histopathological changes were lower in the muscle tissue of Cd+HA group. These results suggest that humic acid may counteract the cadmium toxicity in muscles tissue in histopathologic aspect.