GC-MS Determination of Atenolol Plasma Concentration after Derivatization with N-methyl-N-(trimethylsilyl)trifluoroacetamide

YILMAZ B., ARSLAN S.

CHROMATOGRAPHIA, cilt.70, ss.1399-1404, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1365/s10337-009-1325-3
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1399-1404
  • Anahtar Kelimeler: Gas chromatography-mass spectrometry, Atenolol in plasma, Pharmacokinetics, PERFORMANCE LIQUID-CHROMATOGRAPHY, BETA-BLOCKERS, FLUORESCENCE DETECTION, WHOLE-BLOOD, URINE, ASSAY
  • Atatürk Üniversitesi Adresli: Evet

Özet

An analytical procedure was developed and validated for the determination of atenolol in human plasma. Atenolol and metoprolol (internal standard) were extracted from human plasma with a mixture of chloroform and butanol at basic pH. The extracts were derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide and analyzed by GC-MS. Calibration curves were linear over the concentration range 15-250 ng mL(-1). Intra- and inter-day precision values for atenolol in human plasma were less than 7.4, and accuracy (relative error) was better than 6.4%. Recovery of atenolol from human plasma averaged 90.46%. The limits of detection (LOD) and quantitation (LOQ) of atenolol were 5.0 and 15 ng mL(-1). This method was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol.