Chicken feather protein hydrolysate improves cold resistance by upregulating physiologic and biochemical responses of wheat (Triticum aestivum L.)

Gezgincioglu E., ATICI Ö.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.30, sa.2, ss.3593-3605, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s11356-022-22013-z
  • Dergi Adı: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3593-3605
  • Anahtar Kelimeler: Chicken feather, Cold stress, Protein hydrolysate, Rubisco, Antioxidant, Wheat, AMINO-ACIDS, ANTIOXIDANT ENZYMES, SEED VIGOR, MAIZE, BIOSTIMULANT, GROWTH, COMBINATION, GLUTATHIONE, PEROXIDASE, TOLERANCE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Chicken feather (CF) is one of the largest by-products of the poultry industry and millions of tons of feathers from poultry processing plants have caused a serious waste issue in almost every country. We produced a chicken feather protein hydrolysate (CFPH) by an alkaline process and investigated its effect on the low-temperature response of two wheat cultivars (Triticum aestivum L., cvs. Altindane and Bezostaja). The CFPH contained 19 proteinogenic and 3 non-proteinogenic amino acids, as well as beneficial salts for plant growth. The aqueous solution of CFPH (0.1%, w/v) was applied to seedling leaves before cold stress and then the seedlings (treated and untreated) were transferred to cold conditions (5/2 degrees C, day/night) for 3 days. The CFPH application increased the expression of Rubisco protein and the contents of photosynthetic pigment, soluble sugar, and free proline while decreasing phenolic content in the leaves of both cultivars under cold stress. The cold application alone increased the levels of reactive oxygen species (ROS) and lipid peroxidation (as malondialdehyde), while CFPH decreased their levels. Compared to cold alone, CFPH stimulated antioxidant enzyme activities in both cultivars. This finding was supported by the changes in isoenzyme profiles of the same enzymes on native PAGE. In addition, CFPH application raised reduced ascorbate and glutathione levels, while decreasing the levels of their oxidized forms. The results showed that the application of waste CF-derived CFPH to leaves as a biostimulant alleviated physiological and antioxidative responses in the wheat seedlings under cold stress, thus having the potential to increase cold-stress tolerance in wheat.